Coding

Part:BBa_K820014

Designed by: Philipp N. Sander   Group: iGEM12_Bonn   (2012-09-26)


mazF-TT

This part encodes for the bacterial endoribonuclease mazF plus a TT double terminator.

MazF is a ACA-specific ribonuclease from B. subtilis which in nature is used for toxin/antitoxin addiction systems. It leads to programmed cell death in bacteria upon high-scale expression, thus qualifying it for use in cell death devices.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None